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m pneumoniae m129 standard strain  (ATCC)


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    Structured Review

    ATCC m pneumoniae m129 standard strain
    Bioinformatics Analysis of M. <t>pneumoniae</t> P1C1079–1518. (A) Prediction of the secondary structure features of P1C1079–1518. (B) Prediction of the tertiary structure of P1C1079–1518. (C) Minimum free energy (MFE) secondary structure of mRNA encoding P1C1079–1518. (D) Centroid secondary structure of mRNA encoding P1C1079–1518.
    M Pneumoniae M129 Standard Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 225 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m pneumoniae m129 standard strain/product/ATCC
    Average 96 stars, based on 225 article reviews
    m pneumoniae m129 standard strain - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "Protective immunity induced by a novel P1 adhesin C-terminal anchored mRNA vaccine against Mycoplasma pneumoniae infection in BALB/c mice"

    Article Title: Protective immunity induced by a novel P1 adhesin C-terminal anchored mRNA vaccine against Mycoplasma pneumoniae infection in BALB/c mice

    Journal: Microbiology Spectrum

    doi: 10.1128/spectrum.02140-24

    Bioinformatics Analysis of M. pneumoniae P1C1079–1518. (A) Prediction of the secondary structure features of P1C1079–1518. (B) Prediction of the tertiary structure of P1C1079–1518. (C) Minimum free energy (MFE) secondary structure of mRNA encoding P1C1079–1518. (D) Centroid secondary structure of mRNA encoding P1C1079–1518.
    Figure Legend Snippet: Bioinformatics Analysis of M. pneumoniae P1C1079–1518. (A) Prediction of the secondary structure features of P1C1079–1518. (B) Prediction of the tertiary structure of P1C1079–1518. (C) Minimum free energy (MFE) secondary structure of mRNA encoding P1C1079–1518. (D) Centroid secondary structure of mRNA encoding P1C1079–1518.

    Techniques Used:

    Construction, expression, purification, and identification of pET-28a(+)/RP1C1079–1518. (A) 0.8% agarose gel electrophoresis: Lane 1 shows the double digestion electrophoresis, and Lane 2 shows the original plasmid electrophoresis. (B) Coomassie brilliant blue analysis of RP1C1079–1518 separated by 12.5% SDS-PAGE: Lanes 1–9 represent RP1C 1079–1518 eluted with 80 mM imidazole wash buffer, with an expected size of approximately 47 kDa. (C) Western blot analysis of M. pneumoniae RP1C 1079–1518 using anti- M . pneumoniae antibody. (D). Western blot analysis of M. pneumoniae RP1C 1079–1518 using anti-His antibody.
    Figure Legend Snippet: Construction, expression, purification, and identification of pET-28a(+)/RP1C1079–1518. (A) 0.8% agarose gel electrophoresis: Lane 1 shows the double digestion electrophoresis, and Lane 2 shows the original plasmid electrophoresis. (B) Coomassie brilliant blue analysis of RP1C1079–1518 separated by 12.5% SDS-PAGE: Lanes 1–9 represent RP1C 1079–1518 eluted with 80 mM imidazole wash buffer, with an expected size of approximately 47 kDa. (C) Western blot analysis of M. pneumoniae RP1C 1079–1518 using anti- M . pneumoniae antibody. (D). Western blot analysis of M. pneumoniae RP1C 1079–1518 using anti-His antibody.

    Techniques Used: Expressing, Purification, Agarose Gel Electrophoresis, Electrophoresis, Plasmid Preparation, SDS Page, Western Blot

    Detection of lung tissue changes in mice on the 7th day post M. pneumoniae challenge. (A) Unstained and Dienes-Stained M. pneumoniae on PPLO Agar. (B) H&E-stained lung tissue sections of mice from different groups post M. pneumoniae challenge. (C) Pathology score of lung tissue sections from mice. (D) M. pneumoniae DNA load in lung tissue homogenates of mice. (E) M. pneumoniae DNA copy numbers. Data are presented as mean ± 95% CI. Statistical significance tested by one-way ANOVA test (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).
    Figure Legend Snippet: Detection of lung tissue changes in mice on the 7th day post M. pneumoniae challenge. (A) Unstained and Dienes-Stained M. pneumoniae on PPLO Agar. (B) H&E-stained lung tissue sections of mice from different groups post M. pneumoniae challenge. (C) Pathology score of lung tissue sections from mice. (D) M. pneumoniae DNA load in lung tissue homogenates of mice. (E) M. pneumoniae DNA copy numbers. Data are presented as mean ± 95% CI. Statistical significance tested by one-way ANOVA test (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

    Techniques Used: Staining

    Cytokine detection in lung tissue homogenate supernatants post- M. pneumoniae challenge. (A) Detection of IL-6 in lung tissue homogenate supernatants. (B) Detection of IL-4 in lung tissue homogenate supernatants. (C) Detection of IFN-γ in lung tissue homogenate supernatants. (D) Detection of IL-10 in lung tissue homogenate supernatants. Data are presented as mean ± 95% CI. Statistical significance tested by one-way ANOVA test (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).
    Figure Legend Snippet: Cytokine detection in lung tissue homogenate supernatants post- M. pneumoniae challenge. (A) Detection of IL-6 in lung tissue homogenate supernatants. (B) Detection of IL-4 in lung tissue homogenate supernatants. (C) Detection of IFN-γ in lung tissue homogenate supernatants. (D) Detection of IL-10 in lung tissue homogenate supernatants. Data are presented as mean ± 95% CI. Statistical significance tested by one-way ANOVA test (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

    Techniques Used:



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    Bioinformatics Analysis of M. <t>pneumoniae</t> P1C1079–1518. (A) Prediction of the secondary structure features of P1C1079–1518. (B) Prediction of the tertiary structure of P1C1079–1518. (C) Minimum free energy (MFE) secondary structure of mRNA encoding P1C1079–1518. (D) Centroid secondary structure of mRNA encoding P1C1079–1518.
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    Bioinformatics Analysis of M. <t>pneumoniae</t> P1C1079–1518. (A) Prediction of the secondary structure features of P1C1079–1518. (B) Prediction of the tertiary structure of P1C1079–1518. (C) Minimum free energy (MFE) secondary structure of mRNA encoding P1C1079–1518. (D) Centroid secondary structure of mRNA encoding P1C1079–1518.
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    Image Search Results


    Bioinformatics Analysis of M. pneumoniae P1C1079–1518. (A) Prediction of the secondary structure features of P1C1079–1518. (B) Prediction of the tertiary structure of P1C1079–1518. (C) Minimum free energy (MFE) secondary structure of mRNA encoding P1C1079–1518. (D) Centroid secondary structure of mRNA encoding P1C1079–1518.

    Journal: Microbiology Spectrum

    Article Title: Protective immunity induced by a novel P1 adhesin C-terminal anchored mRNA vaccine against Mycoplasma pneumoniae infection in BALB/c mice

    doi: 10.1128/spectrum.02140-24

    Figure Lengend Snippet: Bioinformatics Analysis of M. pneumoniae P1C1079–1518. (A) Prediction of the secondary structure features of P1C1079–1518. (B) Prediction of the tertiary structure of P1C1079–1518. (C) Minimum free energy (MFE) secondary structure of mRNA encoding P1C1079–1518. (D) Centroid secondary structure of mRNA encoding P1C1079–1518.

    Article Snippet: The genomic DNA of the M. pneumoniae M129 standard strain (ATCC 29342) was used as a template to design primers.

    Techniques:

    Construction, expression, purification, and identification of pET-28a(+)/RP1C1079–1518. (A) 0.8% agarose gel electrophoresis: Lane 1 shows the double digestion electrophoresis, and Lane 2 shows the original plasmid electrophoresis. (B) Coomassie brilliant blue analysis of RP1C1079–1518 separated by 12.5% SDS-PAGE: Lanes 1–9 represent RP1C 1079–1518 eluted with 80 mM imidazole wash buffer, with an expected size of approximately 47 kDa. (C) Western blot analysis of M. pneumoniae RP1C 1079–1518 using anti- M . pneumoniae antibody. (D). Western blot analysis of M. pneumoniae RP1C 1079–1518 using anti-His antibody.

    Journal: Microbiology Spectrum

    Article Title: Protective immunity induced by a novel P1 adhesin C-terminal anchored mRNA vaccine against Mycoplasma pneumoniae infection in BALB/c mice

    doi: 10.1128/spectrum.02140-24

    Figure Lengend Snippet: Construction, expression, purification, and identification of pET-28a(+)/RP1C1079–1518. (A) 0.8% agarose gel electrophoresis: Lane 1 shows the double digestion electrophoresis, and Lane 2 shows the original plasmid electrophoresis. (B) Coomassie brilliant blue analysis of RP1C1079–1518 separated by 12.5% SDS-PAGE: Lanes 1–9 represent RP1C 1079–1518 eluted with 80 mM imidazole wash buffer, with an expected size of approximately 47 kDa. (C) Western blot analysis of M. pneumoniae RP1C 1079–1518 using anti- M . pneumoniae antibody. (D). Western blot analysis of M. pneumoniae RP1C 1079–1518 using anti-His antibody.

    Article Snippet: The genomic DNA of the M. pneumoniae M129 standard strain (ATCC 29342) was used as a template to design primers.

    Techniques: Expressing, Purification, Agarose Gel Electrophoresis, Electrophoresis, Plasmid Preparation, SDS Page, Western Blot

    Detection of lung tissue changes in mice on the 7th day post M. pneumoniae challenge. (A) Unstained and Dienes-Stained M. pneumoniae on PPLO Agar. (B) H&E-stained lung tissue sections of mice from different groups post M. pneumoniae challenge. (C) Pathology score of lung tissue sections from mice. (D) M. pneumoniae DNA load in lung tissue homogenates of mice. (E) M. pneumoniae DNA copy numbers. Data are presented as mean ± 95% CI. Statistical significance tested by one-way ANOVA test (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

    Journal: Microbiology Spectrum

    Article Title: Protective immunity induced by a novel P1 adhesin C-terminal anchored mRNA vaccine against Mycoplasma pneumoniae infection in BALB/c mice

    doi: 10.1128/spectrum.02140-24

    Figure Lengend Snippet: Detection of lung tissue changes in mice on the 7th day post M. pneumoniae challenge. (A) Unstained and Dienes-Stained M. pneumoniae on PPLO Agar. (B) H&E-stained lung tissue sections of mice from different groups post M. pneumoniae challenge. (C) Pathology score of lung tissue sections from mice. (D) M. pneumoniae DNA load in lung tissue homogenates of mice. (E) M. pneumoniae DNA copy numbers. Data are presented as mean ± 95% CI. Statistical significance tested by one-way ANOVA test (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

    Article Snippet: The genomic DNA of the M. pneumoniae M129 standard strain (ATCC 29342) was used as a template to design primers.

    Techniques: Staining

    Cytokine detection in lung tissue homogenate supernatants post- M. pneumoniae challenge. (A) Detection of IL-6 in lung tissue homogenate supernatants. (B) Detection of IL-4 in lung tissue homogenate supernatants. (C) Detection of IFN-γ in lung tissue homogenate supernatants. (D) Detection of IL-10 in lung tissue homogenate supernatants. Data are presented as mean ± 95% CI. Statistical significance tested by one-way ANOVA test (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

    Journal: Microbiology Spectrum

    Article Title: Protective immunity induced by a novel P1 adhesin C-terminal anchored mRNA vaccine against Mycoplasma pneumoniae infection in BALB/c mice

    doi: 10.1128/spectrum.02140-24

    Figure Lengend Snippet: Cytokine detection in lung tissue homogenate supernatants post- M. pneumoniae challenge. (A) Detection of IL-6 in lung tissue homogenate supernatants. (B) Detection of IL-4 in lung tissue homogenate supernatants. (C) Detection of IFN-γ in lung tissue homogenate supernatants. (D) Detection of IL-10 in lung tissue homogenate supernatants. Data are presented as mean ± 95% CI. Statistical significance tested by one-way ANOVA test (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

    Article Snippet: The genomic DNA of the M. pneumoniae M129 standard strain (ATCC 29342) was used as a template to design primers.

    Techniques: